Difference between revisions of "Part:BBa K4335002"

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        <img src="https://static.igem.wiki/teams/4335/wiki/staygoldfzx1.png" width="100%" style="float:center">
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        <p style="font-size:1rem">StayGold and other 15 kinds of fluorescent protein fluorescence intensity changes with time
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StayGold has an order of magnitude more light stability than any currently available fluorescent protein and has a cell brightness similar to that of mNeonGreen. Compared with the 15 fluorescent proteins, StayGold showed good photostability.
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Revision as of 03:09, 11 October 2022


StayGold, a newly discoverd green fluorescent protein

StayGold, a green fluorescent protein (GFP) , fluoresces in expression. StayGold has an order of magnitude more photostability than any currently available fluorescent protein. Commonly used to label and trace cellular components. BBa_K4335002 is our StayGold gene optimized for the codon of Chlamydomonas reinhardtii.

Additional Materials

StayGold was extracted from Cytaeis uchidae by Atsushi Miyawaki et al. by the RIKEN Center for Brain Science Research on April 25, 2022.

Absorption spectrum and emission spectrum of StayGold.[Image origin]

StayGold and other 15 kinds of fluorescent protein fluorescence intensity changes with time

StayGold has an order of magnitude more light stability than any currently available fluorescent protein and has a cell brightness similar to that of mNeonGreen. Compared with the 15 fluorescent proteins, StayGold showed good photostability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]