Difference between revisions of "Part:BBa K4335002"

 
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Here we present StayGold, a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. StayGold is over one order of magnitude more photostable than any currently available fluorescent protein and has a cellular brightness similar to mNeonGreen. Researchers used StayGold to image the dynamics of the endoplasmic reticulum (ER) with high spatiotemporal resolution over several minutes using structured illumination microscopy (SIM) and observed substantially less photobleaching than with a GFP variant optimized for stability in the ER. StayGold will substantially reduce the limitations imposed by photobleaching, especially in the live cell or volumetric imaging. We chose StayGold as our reporter fluorescent protein because it has an order of magnitude greater photostability than any currently available fluorescent protein, and the excitation and emission wavelengths of Staygold do not overlap with the chloroplast fluorescence of Chlamydomonas reinhardtii itself
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StayGold, a green fluorescent protein (GFP) , fluoresces in expression. StayGold has an order of magnitude more photostability than any currently available fluorescent protein. Commonly used to label and trace cellular components. BBa_K4335002 is our StayGold gene optimized for the codon of Chlamydomonas reinhardtii.
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<h2>Additional Materials</h2>
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StayGold was extracted from <i>Cytaeis uchidae </i> by Atsushi Miyawaki et al. by the RIKEN Center for Brain Science Research on April 25, 2022.  
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<figure>
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        <img src="https://static.igem.wiki/teams/4335/wiki/staygoldfzx0.png" width="100%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">Absorption spectrum and emission spectrum of StayGold.<a href="https://www.fpbase.org/protein/staygold/">[Image origin]</a>
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        </p>
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        </figcaption>
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    </figure>
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Revision as of 03:04, 11 October 2022


StayGold, a newly discoverd green fluorescent protein

StayGold, a green fluorescent protein (GFP) , fluoresces in expression. StayGold has an order of magnitude more photostability than any currently available fluorescent protein. Commonly used to label and trace cellular components. BBa_K4335002 is our StayGold gene optimized for the codon of Chlamydomonas reinhardtii.

Additional Materials

StayGold was extracted from Cytaeis uchidae by Atsushi Miyawaki et al. by the RIKEN Center for Brain Science Research on April 25, 2022.

Absorption spectrum and emission spectrum of StayGold.[Image origin]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]