Difference between revisions of "Part:BBa K4245136"

 
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<partinfo>BBa_K4245136 short</partinfo>
 
<partinfo>BBa_K4245136 short</partinfo>
  
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BBa_K4245136
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This part is the left component of the split fluorescent DNA aptamer Lettuce (<partinfo>BBa_K4245133</partinfo>), developed by the Weill-Cornell Medical College. Lettuce left is a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).
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The experimentation with this version of Lettuce left was not successful for Lambert iGEM.
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<br>
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<b>References</b>
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VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893
  
 
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Revision as of 01:40, 11 October 2022


Lettuce Left

This part is the left component of the split fluorescent DNA aptamer Lettuce (BBa_K4245133), developed by the Weill-Cornell Medical College. Lettuce left is a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (BBa_K4245135), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).

The experimentation with this version of Lettuce left was not successful for Lambert iGEM.



References
VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]