Difference between revisions of "Part:BBa K4245135:Experience"
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| − | + | The Lettuce split aptamer has been tested with RCP produced through Rolling Circle Amplification (RCA). RCA was run with hsa-miR-1-3p RCA Padlock Probe <partinfo>BBa_K4245200</partinfo>. The products, split Lettuce, and DFHBI-1T were mixed and heated at 70°C for 5 minutes, then cooled and held at 41°C for 1 hour. The fluorescence before and after were measured on the plate reader. (see Fig. 1) | |
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| + | [[File:BBa_K4245134_RCP_Lettuce_Reactions.jpg|thumb|center|500px|<i>Figure 1. Graph of split Lettuce reaction with RCP. The values represent the change in fluorescence before and after the reaction with DFHBI-1T took place.</i>]] | ||
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| + | The increase in fluorescence of the RCP + Lettuce + dye was significantly greater than the controls, which suggests that the split Lettuce was successfully bound to the RCP and induced fluorescence in DFHBI-1T. | ||
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===Applications of BBa_K4245135=== | ===Applications of BBa_K4245135=== | ||
Revision as of 01:39, 11 October 2022
The Lettuce split aptamer has been tested with RCP produced through Rolling Circle Amplification (RCA). RCA was run with hsa-miR-1-3p RCA Padlock Probe BBa_K4245200. The products, split Lettuce, and DFHBI-1T were mixed and heated at 70°C for 5 minutes, then cooled and held at 41°C for 1 hour. The fluorescence before and after were measured on the plate reader. (see Fig. 1)
The increase in fluorescence of the RCP + Lettuce + dye was significantly greater than the controls, which suggests that the split Lettuce was successfully bound to the RCP and induced fluorescence in DFHBI-1T.
Applications of BBa_K4245135
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