Difference between revisions of "Part:BBa K4245135:Design"

 
 
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__NOTOC__
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===Design Notes===
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<partinfo>BBa_K4245135 short</partinfo>
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The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the right portion of the target— the middle sequence complement within the Rolling Circle Product— was added to the 5’ end of the Lettuce right sequence.
  
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<partinfo>BBa_K4245135 SequenceAndFeatures</partinfo>
 
  
  
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===Design Notes===
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===Source===
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Change ME
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The part was acquired as DNA oligos from Integrated DNA Technologies.
  
  
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===Source===
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__NOTOC__
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<partinfo>BBa_K4245135 short</partinfo>
  
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Change ME
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<partinfo>BBa_K4245135 SequenceAndFeatures</partinfo>
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===References===
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Latest revision as of 01:38, 11 October 2022

Design Notes

The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the right portion of the target— the middle sequence complement within the Rolling Circle Product— was added to the 5’ end of the Lettuce right sequence.


Source

The part was acquired as DNA oligos from Integrated DNA Technologies.


Lettuce Right


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]