Difference between revisions of "Part:BBa K4245135"

Revision as of 01:38, 11 October 2022

Lettuce Right

This part is the right component of the split fluorescent DNA aptamer Lettuce BBa_K4245133, developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left BBa_K4245134, induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).

References

VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 9
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 9
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 9
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 9
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4245135 short</partinfo>
-Change ME
+This part is the right component of the split fluorescent DNA aptamer Lettuce <partinfo>BBa_K4245133</partinfo>, developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left <partinfo>BBa_K4245134</partinfo>, induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).
+<b>References</b>
+VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893
 <!-- Add more about the biology of this part here