Difference between revisions of "Part:BBa K4245134"

Revision as of 01:35, 11 October 2022

Modified Lettuce Left

This part is the left component of the split fluorescent DNA aptamer Lettuce (BBa_K4245133), developed by the Weill-Cornell Medical College. Lettuce left is a 43 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (BBa_K4245135), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).

References
VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4245134 short</partinfo>
-Change ME
+This part is the left component of the split fluorescent DNA aptamer Lettuce (<partinfo>BBa_K4245133</partinfo>), developed by the Weill-Cornell Medical College. Lettuce left is a 43 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).
+<b> References </b>
+<br>
+VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893
 <!-- Add more about the biology of this part here