Difference between revisions of "Part:BBa K4245133:Experience"

Revision as of 01:32, 11 October 2022

The Lettuce DNA Aptamer has been tested with DFHBI-1T. The Method adopted by researchers at the Jaffrey Lab was used, and the fluorescence was read on the plate reader. (see Fig. 1)

Figure 1. Graph of fluorescence after DFHBI-1T was added.

The fluorescence of the reaction between the Lettuce aptamer and DFHBI-1T, as seen in Figure 1, appear to be significantly greater than the controls, which contained water, dye, and buffer, as used in the original research. This proved the functionality of the Lettuce DNA fluorescent aptamer, so Lambert iGEM continued with the design and use of the split Lettuce aptamer to better fit the reporting mechanism used in conjunction with Rolling Circle Amplification (RCA).

Applications of BBa_K4245133

User Reviews

UNIQ6381c8abb190cf8d-partinfo-00000000-QINU UNIQ6381c8abb190cf8d-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
+The Lettuce DNA Aptamer has been tested with DFHBI-1T. The Method adopted by researchers at the Jaffrey Lab was used, and the fluorescence was read on the plate reader. (see Fig. 1)
+[[File:BBa_K4245133_Lettuce_Plus_DFHBI-1T.jpg|thumb|center|500px|<i>Figure 1. Graph of fluorescence after DFHBI-1T was added.</i>]]
+The fluorescence of the reaction between the Lettuce aptamer and DFHBI-1T, as seen in Figure 1, appear to be significantly greater than the controls, which contained water, dye, and buffer, as used in the original research. This proved the functionality of the Lettuce DNA fluorescent aptamer, so Lambert iGEM continued with the design and use of the split Lettuce aptamer to better fit the reporting mechanism used in conjunction with Rolling Circle Amplification (RCA).
 ===Applications of BBa_K4245133===