Difference between revisions of "Part:BBa K4245133"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4245133 short</partinfo> | <partinfo>BBa_K4245133 short</partinfo> | ||
| − | + | This part is the full sequence of the fluorescent DNA aptamer Lettuce, developed by the Weill-Cornell Medical College. Lettuce is a 99 nucleotide long DNA aptamer that induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022). | |
| + | <br> | ||
| + | |||
| + | This part can be split into a left (<partinfo>BBa_K4245134</partinfo>) and right (<partinfo>BBa_K4245135</partinfo>) component so that fluorescence is induced when the two components come together in the presence of a target sequence (VarnBuhler et al., 2022). The fluorescence-inducing main sequence of Lettuce is kept the same while flanking sequences are added to the left and right components depending on the target sequence (VarnBuhler et al., 2022). | ||
| + | |||
| + | <br> | ||
| + | VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:26, 11 October 2022
Lettuce Aptamer
This part is the full sequence of the fluorescent DNA aptamer Lettuce, developed by the Weill-Cornell Medical College. Lettuce is a 99 nucleotide long DNA aptamer that induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).
This part can be split into a left (BBa_K4245134) and right (BBa_K4245135) component so that fluorescence is induced when the two components come together in the presence of a target sequence (VarnBuhler et al., 2022). The fluorescence-inducing main sequence of Lettuce is kept the same while flanking sequences are added to the left and right components depending on the target sequence (VarnBuhler et al., 2022).
VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]