Difference between revisions of "Part:BBa K176172:Design"

 
 
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−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176172 short</partinfo>
 
<partinfo>BBa_K176172 short</partinfo>
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Input1 PoPS generates TetR-LVA
 
Input1 PoPS generates TetR-LVA
  
−
pCon 0.04(J23109) generates LuxR
+
pCon 0.15(K176008) generates LuxR
  
 
pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc  
 
pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc  
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===Source===
 
===Source===
  
−
K176006 K176130
+
K176006 K176128
  
 
===References===
 
===References===

Latest revision as of 08:39, 7 October 2009

Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1833
    Illegal BsaI.rc site found at 2577


Design Notes

Input1: PoPS

Input2: AHL

Output: GFP

Design:

Input1 PoPS generates TetR-LVA

pCon 0.15(K176008) generates LuxR

pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc


Source

K176006 K176128

References