Difference between revisions of "Part:BBa K4182008:Design"
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This gene cluster has been optimized for E. coli | This gene cluster has been optimized for E. coli | ||
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===Source=== | ===Source=== | ||
Revision as of 00:44, 11 October 2022
AA cluster
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1877
Illegal EcoRI site found at 4795
Illegal PstI site found at 126
Illegal PstI site found at 306
Illegal PstI site found at 1413
Illegal PstI site found at 2744
Illegal PstI site found at 4552 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1877
Illegal EcoRI site found at 4795
Illegal PstI site found at 126
Illegal PstI site found at 306
Illegal PstI site found at 1413
Illegal PstI site found at 2744
Illegal PstI site found at 4552 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1877
Illegal EcoRI site found at 4795
Illegal BamHI site found at 184 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1877
Illegal EcoRI site found at 4795
Illegal PstI site found at 126
Illegal PstI site found at 306
Illegal PstI site found at 1413
Illegal PstI site found at 2744
Illegal PstI site found at 4552 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1877
Illegal EcoRI site found at 4795
Illegal PstI site found at 126
Illegal PstI site found at 306
Illegal PstI site found at 1413
Illegal PstI site found at 2744
Illegal PstI site found at 4552
Illegal NgoMIV site found at 2015
Illegal NgoMIV site found at 3345
Illegal AgeI site found at 2170
Illegal AgeI site found at 3970 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This gene cluster has been optimized for E. coli
Source
LacI:E.coli FPPS:Rhodobacter azotoformans fpps gene for farnesyl diphosphate synthase, partial cds(GenBank: AB053174.1)