Difference between revisions of "Part:BBa K176170:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176170 short</partinfo>
 
<partinfo>BBa_K176170 short</partinfo>
Line 13: Line 12:
 
Design:
 
Design:
  
−
pCon 0.70(K176009) generates LuxR
+
pCon 0.70(J23101) generates LuxR
  
 
pLux/Tet(K176000) generates lacZalpha-ccdB-LVA (weak RBS) to kill the cell, activated by LuxR and AHL, repressed by TetR and no aTc
 
pLux/Tet(K176000) generates lacZalpha-ccdB-LVA (weak RBS) to kill the cell, activated by LuxR and AHL, repressed by TetR and no aTc

Latest revision as of 10:24, 6 October 2009

TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1474
    Illegal BsaI.rc site found at 985


Design Notes

Input: AHL

Output: cell death

Design:

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates lacZalpha-ccdB-LVA (weak RBS) to kill the cell, activated by LuxR and AHL, repressed by TetR and no aTc


Source

K176028 K176166

References