Difference between revisions of "Part:BBa K4260011:Design"

(Source)
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
No considerations were necessary.  
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Codon optimization was performed for both coding sequences.
 
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===Source===
 
===Source===

Latest revision as of 21:26, 10 October 2022


Coding sequence for Isoeugenol Monooxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 66
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 1382
    Illegal AgeI site found at 598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694


Design Notes

Codon optimization was performed for both coding sequences.

Source

The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.

References