Difference between revisions of "Part:BBa J428351"

(→‎Cambridge 2022 DV Characterisation)
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<ul>
 
<ul>
 
   <li>Summary</li>
 
   <li>Summary</li>
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As part of our Bronze - Contribution criteria, we characterised 3 JUMP DV plasmids, from the 2022 distribution kit plate 1, against increasing concentrations of ammonium sulphate. These were of varying copy number: pSC101 (low copy no.), pBBR1 (low-med. copy no.) and pUC (high copy no.) to investigate the effect of ammonium on different copy number burden.
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DV pSC101 was the first cloning vector to be created and also gave us the most interesting results so we are focusing our characterisation on this one. Low copy number plasmids are often used as default so this allows us to made a larger contribution to the iGEM and synthetic biologist community.
 +
 
   <li>Verdict</li>
 
   <li>Verdict</li>
 
   <li>Experience/Detailed Protocol: Link here</li>
 
   <li>Experience/Detailed Protocol: Link here</li>

Revision as of 20:15, 10 October 2022

pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number)


Characterisation Studies

Cambridge 2022 JUMP DV Characterisation

  • Summary

    • As part of our Bronze - Contribution criteria, we characterised 3 JUMP DV plasmids, from the 2022 distribution kit plate 1, against increasing concentrations of ammonium sulphate. These were of varying copy number: pSC101 (low copy no.), pBBR1 (low-med. copy no.) and pUC (high copy no.) to investigate the effect of ammonium on different copy number burden.

    DV pSC101 was the first cloning vector to be created and also gave us the most interesting results so we are focusing our characterisation on this one. Low copy number plasmids are often used as default so this allows us to made a larger contribution to the iGEM and synthetic biologist community.

  • Verdict
  • Experience/Detailed Protocol: Link here

DNA Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3023
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 3029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3023
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3023
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3038
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.