Difference between revisions of "Part:BBa K4195103"

 
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===Usage and Biology===
 
===Usage and Biology===
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ClyA
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Cytolysin A (ClyA) is a pore-forming toxin that is produced by some bacteria from the <i>Enterobacteriaceae</i> family. When fused to the C-terminal of ClyA, heterologous proteins can be displayed on the surface of the engineered bacteria and OMVs (outer membrane vesicles) released by them (<i>1</i>).
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TTPB
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TTPB is tail tubular protein B of podophage 7. It has been found that TTPB serves as ligands that recognizes the conserved <i>Vibrio</i> receptor Vp0980 to mediate phage adsorption. It binds with Vp0980 of <i>Vibrio parahaemolyticus</i> and then mediates phage adsorption and subsequent bacterial lysis (<i>2</i>).
  
 
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Revision as of 17:23, 10 October 2022


I0500-B0034-clyA-ttpB-B0015

Usage and Biology

ClyA Cytolysin A (ClyA) is a pore-forming toxin that is produced by some bacteria from the Enterobacteriaceae family. When fused to the C-terminal of ClyA, heterologous proteins can be displayed on the surface of the engineered bacteria and OMVs (outer membrane vesicles) released by them (1). TTPB TTPB is tail tubular protein B of podophage 7. It has been found that TTPB serves as ligands that recognizes the conserved Vibrio receptor Vp0980 to mediate phage adsorption. It binds with Vp0980 of Vibrio parahaemolyticus and then mediates phage adsorption and subsequent bacterial lysis (2).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1561
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3402
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2446
    Illegal AgeI site found at 3874
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2008
    Illegal SapI site found at 961