Difference between revisions of "Part:BBa K4375007"
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INPNC codes for N- and C- terminal domain of Ice Nucleation Protein (INP) from Pseudomonas syringae, and it can be used for displaying proteins on bacteria's outer membrane.
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INPNC codes for N- and C- terminal domain of Ice Nucleation Protein (INP) from ''Pseudomonas syringae'', and it can be used for displaying proteins on bacteria's outer membrane.
  
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==Usage and Biology==
 
==Usage and Biology==
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The INP (Ice Nucleation Protein) is a membrane-bound protein from bacteria, like Pseudomonas and Erwinia, and can be used as an anchor motif for cell surface display. INPNC contains the N -and C-terminal domains of this protein in a truncated form. Through codon optimization, we made it compatible with ''E.Coli'' to be expressed.
  
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The INP (Ice Nucleation Protein) is a membrane-bound protein from bacteria, like Pseudomonas and Erwinia, and can be used as an anchor motif for cell surface display. INPNC contains the N -and C-terminal domains of this protein in a truncated form. Through codon optimization, we made it compatible with E.Coli to be expressed.
 
  
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==Sequence and Features==
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4375007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4375007 SequenceAndFeatures</partinfo>
  
  
 
==Reference==
 
==Reference==
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Wu, P.-H.; Giridhar, R.; Wu, W.-T. Surface Display of Transglucosidase onEscherichia Coli by Using the Ice Nucleation Protein ofXanthomonas Campestris and Its Application in Glucosylation of Hydroquinone. Biotechnology and Bioengineering 2006, 95 (6), 1138–1147. https://doi.org/10.1002/bit.21076.
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Wu PH, Giridhar R, Wu WT. Surface display of transglucosidase on Escherichia coli by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. Biotechnology and bioengineering. 2006 Dec 20;95(6):1138-47.
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Revision as of 16:48, 10 October 2022


Codon optimalised INPNC for Surface Display

INPNC codes for N- and C- terminal domain of Ice Nucleation Protein (INP) from Pseudomonas syringae, and it can be used for displaying proteins on bacteria's outer membrane.



Usage and Biology

The INP (Ice Nucleation Protein) is a membrane-bound protein from bacteria, like Pseudomonas and Erwinia, and can be used as an anchor motif for cell surface display. INPNC contains the N -and C-terminal domains of this protein in a truncated form. Through codon optimization, we made it compatible with E.Coli to be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Wu, P.-H.; Giridhar, R.; Wu, W.-T. Surface Display of Transglucosidase onEscherichia Coli by Using the Ice Nucleation Protein ofXanthomonas Campestris and Its Application in Glucosylation of Hydroquinone. Biotechnology and Bioengineering 2006, 95 (6), 1138–1147. https://doi.org/10.1002/bit.21076.