Difference between revisions of "Part:BBa K4342000"
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| − | The <i> tdk/kan </i> cassette is a highly versatile sequence used for efficiently and effectively engineering the <i> Acinetobacter baylyi </i> ADP1 genome. This part contains the <i> kanR </i> and <i> tdk </i> genes which allow for the selection and counterselection of genetically engineered ADP1 cells. When this cassette is integrated into the ADP1 genome, the <i> kanR </i> gene confers resistance to kanamycin and the <i> tdk </i> gene is lethal to the cell when Azidothymidine is present (Metzgar et. al - 2004). | + | The <i>tdk/kan</i> cassette is a highly versatile sequence used for efficiently and effectively engineering the <i> Acinetobacter baylyi </i> ADP1 genome. This part contains the <i> kanR </i> and <i> tdk </i> genes which allow for the selection and counterselection of genetically engineered ADP1 cells. When this cassette is integrated into the ADP1 genome, the <i> kanR </i> gene confers resistance to kanamycin and the <i> tdk </i> gene is lethal to the cell when Azidothymidine is present (Metzgar et. al - 2004). |
The 2022 UT Austin iGEM team used the <i> tdk/kan </i> cassette to successfully delete a number of genes, insert DNA sequences into the ADP1 chromosome, and engineer an ADP1-based biosensor for detecting any DNA sequence of interest. The composite parts below utilized the <i> tdk/kan </i> cassette for genetic engineering. | The 2022 UT Austin iGEM team used the <i> tdk/kan </i> cassette to successfully delete a number of genes, insert DNA sequences into the ADP1 chromosome, and engineer an ADP1-based biosensor for detecting any DNA sequence of interest. The composite parts below utilized the <i> tdk/kan </i> cassette for genetic engineering. | ||
Revision as of 16:47, 10 October 2022
tdk/kan Cassette
Usage and Biology
The tdk/kan cassette is a highly versatile sequence used for efficiently and effectively engineering the Acinetobacter baylyi ADP1 genome. This part contains the kanR and tdk genes which allow for the selection and counterselection of genetically engineered ADP1 cells. When this cassette is integrated into the ADP1 genome, the kanR gene confers resistance to kanamycin and the tdk gene is lethal to the cell when Azidothymidine is present (Metzgar et. al - 2004).
The 2022 UT Austin iGEM team used the tdk/kan cassette to successfully delete a number of genes, insert DNA sequences into the ADP1 chromosome, and engineer an ADP1-based biosensor for detecting any DNA sequence of interest. The composite parts below utilized the tdk/kan cassette for genetic engineering.
- ACIAD2049 tdk/kan Cassette
- ACIAD2049 Rescue Cassette
- AcrB tdk/kan Cassette
- AcrB Rescue Cassette
- PbpG tdk/kan Cassette
- PbpG Rescue Cassette
- RecJ tdk/kan Cassette
- RecJ Rescue Cassette
- Broken nptII Gene
- nptII Gene Detector Construct
- TEM-1 Broken Gene
- TEM-1 Gene Detector Construct
- P. destructans Detector Construct