Difference between revisions of "Part:BBa K4244009"
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The chimeric two-component system (TCS) is formed by combining the extracellular Dismed2 domain of the RetS protein from P. aeruginosa and intracellular HisKA and HATPase intracellular domains of EnvZ part of the EnvZ-OmpR TCS. In this system mucin activates the chimeric TCS, which in turns allow the expression of the essential gene <i>gmk</i>.
 
The chimeric two-component system (TCS) is formed by combining the extracellular Dismed2 domain of the RetS protein from P. aeruginosa and intracellular HisKA and HATPase intracellular domains of EnvZ part of the EnvZ-OmpR TCS. In this system mucin activates the chimeric TCS, which in turns allow the expression of the essential gene <i>gmk</i>.
  
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[[file:Dismed2EnvZ.jpeg|500px]]
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[[file:Dismed2EnvZ.jpeg|left|500px]]<br>
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[[File:Dismed2EnvZ_results.jpeg|thumb|500px|center|<font size="1"> Figure 1: GFP fluorescence values of E. coli strain lacking the EnvZ gene after induction with varying concentrations of mucin in M9 medium at 14th hour of incubation. The first negative control is the naked strain, harbouring no plasmid. The second negative control have the Ompc:GFP plasmid (<partinfo>BBa_K4244011</partinfo>). While the chimeric TCS contains both the OmpC:GFP and the chimeric Dismed2 EnvZ (<partinfo>BBa_K4244009</partinfo>). The graph shows that with 5% of mucin the fluorescence of the chimeric TCSis significantly higher than with no mucin.
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The graph shows the GFP fluorescence values of E. coli strain lacking the EnvZ gene after induction with varying concentrations of mucin in M9 medium at 14th hour of incubation. The first negative control is the naked strain, harbouring no plasmid. The second negative control have the Ompc:GFP plasmid (BBa_K4244011). While the chimeric TCS contains both the OmpC:GFP and the chimeric Dismed2 EnvZ (BBa_K4244009). The graph shows that with 5% of mucin the fluorescence of the chimeric TCSis significantly higher than with no mucin.
 
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[[file:Dismed2EnvZ_results.jpeg|500px]]
 
  
 
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Revision as of 16:38, 10 October 2022


Promoter:Dismed2EnvZ:Terminator

The chimeric two-component system (TCS) is formed by combining the extracellular Dismed2 domain of the RetS protein from P. aeruginosa and intracellular HisKA and HATPase intracellular domains of EnvZ part of the EnvZ-OmpR TCS. In this system mucin activates the chimeric TCS, which in turns allow the expression of the essential gene gmk.

Dismed2EnvZ.jpeg



Figure 1: GFP fluorescence values of E. coli strain lacking the EnvZ gene after induction with varying concentrations of mucin in M9 medium at 14th hour of incubation. The first negative control is the naked strain, harbouring no plasmid. The second negative control have the Ompc:GFP plasmid (BBa_K4244011). While the chimeric TCS contains both the OmpC:GFP and the chimeric Dismed2 EnvZ (BBa_K4244009). The graph shows that with 5% of mucin the fluorescence of the chimeric TCSis significantly higher than with no mucin.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 332
    Illegal PstI site found at 865
    Illegal NgoMIV site found at 130
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 596
    Illegal NgoMIV site found at 1063
    Illegal NgoMIV site found at 1103
  • 1000
    COMPATIBLE WITH RFC[1000]