Difference between revisions of "Part:BBa K4335004"

Revision as of 16:33, 10 October 2022 (view source) ZXFeng (Talk | contribs) ← Older edit		Revision as of 16:35, 10 October 2022 (view source) ZXFeng (Talk | contribs) Newer edit →
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	When we looked through the blast tool on the registry page, we found a part		When we looked through the blast tool on the registry page, we found a part
−	<a href="https://parts.igem.org/Part:BBa_K2136016">[BBa_K2136016]</a> that was very similar to ours.	+	<a href="https://parts.igem.org/Part:BBa_K2136016">[BBa_K2136016]</a> that was very similar to ours.<br>
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		+	This is also a codon optimized mCherry for <i>Chlamydomonas reinhardtii</i>.
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Revision as of 16:35, 10 October 2022

mCherry

mCherry is a red fluorescent protein. Fluorescent reporter genes for biological applications. We optimized its codon to express in Chlamydomonas reinhardtii more efficiently.

BBa_K4335004 is the DNA of mCherry gene derived from mushroom coral and it is codon optimized for Chlamydomonas reinhardtii .

The advantage of mcherry over other fluorescent proteins is that its color and green fluorescent protein (GFP) are co-labeled, and mcherry also has excellent light stability over other monomeric fluorescent proteins.

It's believed that codon-choice have been conserved during evolution course and because not all tRNA are expressed equally, specially across species, a particular DNA sequence can be codon optimised to match the most prevalent tRNAs of the host cell, improving the efficiency of protein translation [1].

When we looked through the blast tool on the registry page, we found a part [BBa_K2136016] that was very similar to ours.

This is also a codon optimized mCherry for Chlamydomonas reinhardtii.

Sequence and Features