Difference between revisions of "Part:BBa K4165174"
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===Source===
 
===Source===
 
Synthesized
 
Synthesized
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===<span class='h3bb'>Sequence and Features</span>===
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<partinfo>BBa_K4165174 SequenceAndFeatures</partinfo>
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===Dry Lab===
 
===Dry Lab===
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                 Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                 Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                     variation of mRNA and protein concentrations with time compared with the wet lab results.
 
                     variation of mRNA and protein concentrations with time compared with the wet lab results.
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4165174 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 16:20, 10 October 2022


T7 promoter-GST-H1A-T7 terminator

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), GST tag(BBa_K4165070), H1A peptide (BBa_K4165000), and T7 terminator (BBa_K731721), The GST tag was attached to the H1A coding sequence to serve in the purification using Glutathione resin column.

Usage and Biology

a synthetic peptide that binds to the PDZ domain of HTRA1 protein. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis.

Source

Synthesized

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 173


Dry Lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

               Figure 1. this figure shows the results from the transcription and translation code showing the 
                   variation of mRNA and protein concentrations with time compared with the wet lab results.