Difference between revisions of "Part:BBa K4245002:Design"

Latest revision as of 16:08, 10 October 2022

iSpinach aptamer with LacI repression

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 375
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245003, a composite part producing iSpinach-D5-G30-A32; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245003, a composite part producing iSpinach-D5-G30-A32; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.
+This part works identically to <partinfo>BBa_K4245001</partinfo>, a composite part producing Spinach, and <partinfo>BBa_K4245003</partinfo>, a composite part producing iSpinach-D5-G30-A32; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.
 ===Source===