Difference between revisions of "Part:BBa K4335013"

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		+	<h2>Result</h2>
		+	<h3>Plasmid Construction</h3>
−		+	<h3>Functional Identification</h3>
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Revision as of 15:56, 10 October 2022

SpCas9 The Cas9 protein is part of the bacteria's CRISPR/Cas9 immune defense mechanism to identify and destroy foreign DNA. By incorporating the foreign DNA into the bacteria's own DNA, it has a memory of any prior foreign DNA that the bacteria has encountered.

The Cas9 endonuclease can be used either with the tracrRNA and crRNA (CRISPR RNA- Clustered Regularly Interspaced Short Palindromic Repeats) or with a guide RNA to cleave foreign DNA at a species locations. This makes exceptionally useful for DNA cloning in synthetic biology.

Usage

We found a SpCas9 from iGEM16_Cambridge-JIC in the previous part [BBa_K2148013] .

This part is based on the original Cas9 part in the registry, BBa_K1218011. To enhance the expression of Cas9 in the chloroplast chassis, Cambridge-JIC have codon-optimised it using the codon-optimisation software developed by Saul Purton.

The amino acid and nucleotide sequences of BBa_K2148013 and BBa_K2148013 were compared.

Result

Plasmid Construction

Functional Identification

Sequence and Features