Difference between revisions of "Part:BBa K4244039"

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<partinfo>BBa_K4244039 short</partinfo>
 
<partinfo>BBa_K4244039 short</partinfo>
  
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The P11 promoter was combined with an RBS derived from RBS calculator. In between these a target region was introduced including PAM and the B1 protospacer was introduced, which is targeted by dSpyCas9.
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The P11 promoter was combined with an RBS derived from [https://salislab.net/software/predict_rbs_calculator RBS calculator]<br> In between these a target region was introduced including PAM and the B1 protospacer was introduced, which is targeted by dSpyCas9.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:47, 10 October 2022


P11 + medium RBS

The P11 promoter was combined with an RBS derived from RBS calculator
In between these a target region was introduced including PAM and the B1 protospacer was introduced, which is targeted by dSpyCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 54
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PMID: 23474465.