Difference between revisions of "Part:BBa K4244050"

 
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<partinfo>BBa_K4244050 short</partinfo>
 
<partinfo>BBa_K4244050 short</partinfo>
  
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WIP
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The sequence includes two repetitive sequences recognisable by the Type-IC Cas proteins. The second repetitive sequence is modified to prevent recombination, so the spacer sequence that is placed in between does not get flipped out. The sequence found in the middle of the repeats is a random DNA sequence, that can be substituted by a spacer targeting a desired locus in the genome. BsaI sites can be found at the end of the first repeat and at the beginning of the second, so a new spacer can be integrated through simple restriction-ligation.
  
 
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Revision as of 15:27, 10 October 2022


CRISPR array

The sequence includes two repetitive sequences recognisable by the Type-IC Cas proteins. The second repetitive sequence is modified to prevent recombination, so the spacer sequence that is placed in between does not get flipped out. The sequence found in the middle of the repeats is a random DNA sequence, that can be substituted by a spacer targeting a desired locus in the genome. BsaI sites can be found at the end of the first repeat and at the beginning of the second, so a new spacer can be integrated through simple restriction-ligation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 51
    Illegal BsaI.rc site found at 33