Difference between revisions of "Part:BBa J70503:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70503 short</partinfo> | <partinfo>BBa_J70503 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Needs mutation of EcoRI | + | Needs mutation of EcoRI sites and GATC sites. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 01:29, 4 October 2009
Mesoplasma florum origin of replication
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 928
Illegal EcoRI site found at 1609 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 928
Illegal EcoRI site found at 1609 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 928
Illegal EcoRI site found at 1609
Illegal BglII site found at 466 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 928
Illegal EcoRI site found at 1609 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 928
Illegal EcoRI site found at 1609 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Needs mutation of EcoRI sites and GATC sites.
Source
PCR from genomic DNA