Difference between revisions of "Part:BBa J04450:Experience"

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We've used this part as an alternative to the suicide part p1010 (ccdB) for selecting against cells that have been transformed with a [http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf destination plasmid]. It worked perfectly, and if i might say, it looks smashingly in LB medium ;).
 
We've used this part as an alternative to the suicide part p1010 (ccdB) for selecting against cells that have been transformed with a [http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf destination plasmid]. It worked perfectly, and if i might say, it looks smashingly in LB medium ;).
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<partinfo>BBa_J04450 AddReview 4</partinfo>
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<I>iGEM Groningen 2009</I>
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We transformed E.coli TOP10 cells by mistake with this plasmid, and found perfect red cells after over night growth. The restriction of the plasmid with EcoRI and PstI fast digest enzyme showed the expected fragment sizes. We did not continue work with the part, because it was not part of our cloning and assembling plan. Nice colour anyway!.
 
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Revision as of 19:31, 3 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J04450

User Reviews

UNIQ4dc76e2f8f955210-partinfo-00000000-QINU

•••••

Antiquity

This review comes from the old result system and indicates that this part worked in some test.

•••••

Marc.mtk

We've used this part as an alternative to the suicide part p1010 (ccdB) for selecting against cells that have been transformed with a [http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf destination plasmid]. It worked perfectly, and if i might say, it looks smashingly in LB medium ;).

••••

iGEM Groningen 2009

We transformed E.coli TOP10 cells by mistake with this plasmid, and found perfect red cells after over night growth. The restriction of the plasmid with EcoRI and PstI fast digest enzyme showed the expected fragment sizes. We did not continue work with the part, because it was not part of our cloning and assembling plan. Nice colour anyway!.

UNIQ4dc76e2f8f955210-partinfo-00000004-QINU