Difference between revisions of "Part:BBa K4325003"
Line 2: Line 2:
 
<partinfo>BBa_K4325003 short</partinfo>
 
<partinfo>BBa_K4325003 short</partinfo>
 
===Description===
 
===Description===
−
<i> gshF </i>encodes a bifunctional glutathione synthetase GshF originated from <i> Streptococcus thermophilus</i>  
+
<p>encodes a bifunctional glutathione synthetase GshF originated from <i> Streptococcus thermophilus</i></p>  
 
===Usage===
 
===Usage===
−
<p>it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii. (<partinfo>BBa_K4325003 </partinfo>)
+
<p>it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii.</p>  
  
  

Revision as of 13:46, 10 October 2022

gshF

Description

encodes a bifunctional glutathione synthetase GshF originated from Streptococcus thermophilus

Usage

it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
    Illegal NotI site found at 1838
    Illegal NotI site found at 2038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal XhoI site found at 2019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
    Illegal NgoMIV site found at 1620
    Illegal NgoMIV site found at 2251
  • 1000
    COMPATIBLE WITH RFC[1000]


2022 SZPT-China

Characterization

1. Verification of Western blot electrophoresis in E. coli.

Figure1: Verification of Western blot electrophoresis in E. coli

As shown in Figure 1, the expression of GshF were verified successfully by Western blot.

Reference

Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications.