Difference between revisions of "Part:BBa K4325003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4325003 short</partinfo>
 
<partinfo>BBa_K4325003 short</partinfo>
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===Description===
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<i> gshF </i>encodes a bifunctional glutathione synthetase GshF originated from <i> Streptococcus thermophilus</i>
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===Usage===
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<p>it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii. (<partinfo>BBa_K4325003 </partinfo>)
  
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AAAA
 
  
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<!-- Add more about the biology of this part here
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===Sequence and Features===
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===Usage and Biology===
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<!-- -->
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K4325003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4325003 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4325003 parameters</partinfo>
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<partinfo>BBa_K4325025 parameters</partinfo>
 
<!-- -->
 
<!-- -->
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=2022 SZPT-China=
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<h3>Characterization</h3>
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<h4>
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1. Verification of Western blot electrophoresis in <i> E. coli</i>.</h4>
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[[File:Mfold-K3257013-1.png|600px|thumb|center|Figure1: Verification of Western blot electrophoresis in <i> E. coli</i>]]<p></p> As shown in Figure 1, the expression of GshF were verified successfully by Western blot.
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<h3>Reference</h3>
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<p> Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications.
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</p >

Revision as of 13:43, 10 October 2022

gshF

Description

gshF encodes a bifunctional glutathione synthetase GshF originated from Streptococcus thermophilus

Usage

it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii. (BBa_K4325003)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
    Illegal NotI site found at 1838
    Illegal NotI site found at 2038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal XhoI site found at 2019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 760
    Illegal EcoRI site found at 1195
    Illegal EcoRI site found at 1693
    Illegal PstI site found at 19
    Illegal PstI site found at 49
    Illegal PstI site found at 406
    Illegal NgoMIV site found at 1620
    Illegal NgoMIV site found at 2251
  • 1000
    COMPATIBLE WITH RFC[1000]


2022 SZPT-China

Characterization

1. Verification of Western blot electrophoresis in E. coli.

Figure1: Verification of Western blot electrophoresis in E. coli
<p>

As shown in Figure 1, the expression of GshF were verified successfully by Western blot.

Reference

Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications.