Difference between revisions of "Part:BBa K4195078"
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| − | + | ===Biology=== | |
| − | + | This sequence is the first part of guide designed for detection of toxin gene '''pirB'''. | |
| − | + | Ribozyme ENabled Detection of RNA (RENDR)<br/> | |
| − | + | RENDR is a high-performing, plug-and-play RNA-sensing platform('''1'''). RENDR utilizes a split variant of the '''Tetrahymena thermophila''' ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/> | |
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Revision as of 13:36, 10 October 2022
Biology
This sequence is the first part of guide designed for detection of toxin gene pirB.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.