Difference between revisions of "Part:BBa K4245201"

 
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<partinfo>BBa_K4245201 short</partinfo>
 
<partinfo>BBa_K4245201 short</partinfo>
  
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hsa-miR-1-3p RCT Padlock Probe
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This part is the sequence for the hsa-miR-1-3p RCT Padlock Probe, which our team designed to be specific to hsa-miR-1-3p (<partinfo>BBa_K4245006</partinfo>). This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Transcription approach, the produced transcript contains repeats of the middle sequence (hsa-miR-1-3p, the broccoli fluorescent RNA aptamer, and the padlock spacer sequence). We cloned the sequence into a pSB1K3 backbone.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4245201 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4245201 parameters</partinfo>
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Revision as of 13:26, 10 October 2022


hsa-miR-1-3p RCT Padlock Probe

This part is the sequence for the hsa-miR-1-3p RCT Padlock Probe, which our team designed to be specific to hsa-miR-1-3p (BBa_K4245006). This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Transcription approach, the produced transcript contains repeats of the middle sequence (hsa-miR-1-3p, the broccoli fluorescent RNA aptamer, and the padlock spacer sequence). We cloned the sequence into a pSB1K3 backbone.