Difference between revisions of "Part:BBa K4156076"
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pCadC is regulated by membrane-tethered activator protein (CadC), exhibits higher activity in acidic media than in media at neutral pH. In pH reporter strains, it’s used to test their response to acidic conditions in tumors induction.
 
pCadC is regulated by membrane-tethered activator protein (CadC), exhibits higher activity in acidic media than in media at neutral pH. In pH reporter strains, it’s used to test their response to acidic conditions in tumors induction.
  
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===Characterization===
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=='''pH induced promoter testing'''==
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We constructed a pH reporter consisting of the pH-inducible promoter pCadC+mRFP. To test itsperformance, we added reporter in different chassis organisms. Fig 1,2 illustrates that pCadC induces the expression of the downstream gene mRFP with the decrease of pH,. Thus, it can be seen that pH reporter can work properly.
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/1-1.png" alt="control">
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                <figcaption><b>Figure 1:</b> Induction of downstream gene mRFP expression with different pH values in different chassis organisms over 48h.</figcaption>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/1-2-4-2.png" alt="control">
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                <figcaption><b>Figure 2:</b>  Induction of downstream gene mRFP expression over time by a pH reporter consisting of pCadC+mRFP at different pH values.</figcaption>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:23, 10 October 2022


Promoter pCadC

pCadC is a pH-sensitive promoter, designed to response the low-pH conditions.


Usage and Biology

pCadC is regulated by membrane-tethered activator protein (CadC), exhibits higher activity in acidic media than in media at neutral pH. In pH reporter strains, it’s used to test their response to acidic conditions in tumors induction.

Characterization

pH induced promoter testing

We constructed a pH reporter consisting of the pH-inducible promoter pCadC+mRFP. To test itsperformance, we added reporter in different chassis organisms. Fig 1,2 illustrates that pCadC induces the expression of the downstream gene mRFP with the decrease of pH,. Thus, it can be seen that pH reporter can work properly.

control
Figure 1: Induction of downstream gene mRFP expression with different pH values in different chassis organisms over 48h.


control
Figure 2: Induction of downstream gene mRFP expression over time by a pH reporter consisting of pCadC+mRFP at different pH values.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 358