Difference between revisions of "Part:BBa K4156126:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | To export the HlpA-binding protein to the EcN surface, a (truncated) INP (ice nuclease protein) tag is fused to the N-terminal end of HlpA.Also, we fused 6x his tag at the end of Hlpa to facilitate purification. | |
It is important to note that HlpA is a new part that we uploaded this year. And as a toxic protein, it does not exist in the igem whitelist. We have drafted a safety precaution about HlpA and submitted a check-in form to igem official. The details can be seen on our wiki. | It is important to note that HlpA is a new part that we uploaded this year. And as a toxic protein, it does not exist in the igem whitelist. We have drafted a safety precaution about HlpA and submitted a check-in form to igem official. The details can be seen on our wiki. | ||
Latest revision as of 13:02, 10 October 2022
HlpA with INP tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 154
Illegal NgoMIV site found at 405
Illegal AgeI site found at 733 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To export the HlpA-binding protein to the EcN surface, a (truncated) INP (ice nuclease protein) tag is fused to the N-terminal end of HlpA.Also, we fused 6x his tag at the end of Hlpa to facilitate purification.
It is important to note that HlpA is a new part that we uploaded this year. And as a toxic protein, it does not exist in the igem whitelist. We have drafted a safety precaution about HlpA and submitted a check-in form to igem official. The details can be seen on our wiki.
Source
Streptococcus gallolyticus