Difference between revisions of "Part:BBa K4245160"

Revision as of 12:54, 10 October 2022

Padlock spacer sequence
This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer (BBa_K3380153) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe (BBa_K4245121), hsa-mir-133a-3p RCT Padlock Probe (BBa_K4245201), and hsa-miR-208a-3p RCT Padlock Probe (BBa_K4245208).

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4245160 short</partinfo>
 <br>
-This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer ([https://parts.igem.org/Part:BBa_K3380153 BBa_K3380153]) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245121 BBa_K4245121]), hsa-mir-133a-3p RCT Padlock Probe (([https://parts.igem.org/Part:BBa_K4245201 BBa_K4245201])), and hsa-miR-208a-3p RCT Padlock Probe (([https://parts.igem.org/Part:BBa_K4245208 BBa_K4245208])).
+This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer ([https://parts.igem.org/Part:BBa_K3380153 BBa_K3380153]) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245121 BBa_K4245121]), hsa-mir-133a-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245201 BBa_K4245201]), and hsa-miR-208a-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245208 BBa_K4245208]).
 ===Usage and Biology===