Difference between revisions of "Part:BBa K4378008:Design"

 
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===Design Notes===
 
===Design Notes===
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This expression cassette has been designed with overexpression in BL21 (DE3) E. coli in mind.
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This expression cassette has been designed with overexpression in BL21 (DE3) <i>E. coli</i> in mind.
  
  

Latest revision as of 12:51, 10 October 2022


MHETase expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1421
    Illegal BglII site found at 1736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 420
    Illegal AgeI site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This expression cassette has been designed with overexpression in BL21 (DE3) E. coli in mind.


Source

Composite part, see basic part pages for source.

References