Difference between revisions of "Part:BBa K4165234"

Revision as of 12:06, 10 October 2022

Clamp [16]

Clamp [16] composed of Tau Binding Peptide WWW ( BBa_K4165007) , linker length of GGSGGGG (BBa_K4165018), Tau Binding Peptide TD28rev (BBa_K4165006). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.

Usage and Biology

According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).

Sequence and Features

3D Modeling and Ranking

According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).

                              Figure 1. The 3D structure of Clamp 16 modeled by TRrosetta