Difference between revisions of "Part:BBa K4343141"

 
 
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nSpRY (nick SpRY) is a PAM-restricted Cas9 obtained by directed evolution, while mutating the RuvC structural domain [1].
  
 
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Latest revision as of 12:02, 10 October 2022


nSpRY

nSpRY (nick SpRY) is a PAM-restricted Cas9 obtained by directed evolution, while mutating the RuvC structural domain [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 4124