Difference between revisions of "Part:BBa K4461002"
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The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression.  
 
The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression.  
  
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[[File:NirBDC mRNA expression.png|200px|thumb|left|alt text]]
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[[File:NirBDC mRNA expression.png|200px|thumb|left|This is the nirBDC mRNA expression-Time graph.]]
 
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===Usage and Biology===
 
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:56, 10 October 2022


nirBDC promoter

The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression.

This is the nirBDC mRNA expression-Time graph.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]