Difference between revisions of "Part:BBa K4461002"
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The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression. | The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression. | ||
+ | [[File:NirBDC mRNA expression.png|200px|thumb|left|alt text]] | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 11:55, 10 October 2022
nirBDC promoter
The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]