Difference between revisions of "Part:BBa K4343131"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4343131 short</partinfo>
 
<partinfo>BBa_K4343131 short</partinfo>
  
NONE
+
The sgRNA expression frame consisting of the promoter of the transcribed RNA, the sgRNA scaffold and the corresponding N20 (sgRNA1-N20), which directs CBE-related elements to target TRP1 and complete base editing (C→T).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:54, 10 October 2022


sgRNA1

The sgRNA expression frame consisting of the promoter of the transcribed RNA, the sgRNA scaffold and the corresponding N20 (sgRNA1-N20), which directs CBE-related elements to target TRP1 and complete base editing (C→T).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 365
    Illegal BsaI.rc site found at 629