Difference between revisions of "Part:BBa K4343130"

 
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<partinfo>BBa_K4343130 short</partinfo>
 
<partinfo>BBa_K4343130 short</partinfo>
  
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A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nSpRY. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. More critically, SpRY is a PAM-less Cas protein that can be targeted and edited at any location within a gene. At the same time, it is ensured that the editing efficiency is high enough to obtain mutants.
  
 
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Revision as of 11:52, 10 October 2022


TEFin-nSpRYCBE

A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nSpRY. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. More critically, SpRY is a PAM-less Cas protein that can be targeted and edited at any location within a gene. At the same time, it is ensured that the editing efficiency is high enough to obtain mutants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1538
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4865
    Illegal BamHI site found at 3817
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 429
    Illegal BsaI site found at 5443
    Illegal BsaI.rc site found at 423
    Illegal BsaI.rc site found at 4552
    Illegal BsaI.rc site found at 5707