Difference between revisions of "Part:BBa K4343129"

 
 
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<partinfo>BBa_K4343129 short</partinfo>
 
<partinfo>BBa_K4343129 short</partinfo>
  
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A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 85%.
  
 
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Latest revision as of 11:49, 10 October 2022


GAP-nCBE

A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 85%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1679
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5003
    Illegal BamHI site found at 3958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 570
    Illegal BsaI site found at 5581
    Illegal BsaI.rc site found at 564
    Illegal BsaI.rc site found at 4690
    Illegal BsaI.rc site found at 5845