Difference between revisions of "Part:BBa K4343128:Design"

 
 
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===Design Notes===
 
===Design Notes===
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NONE
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A cytosine base editor with EXP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 70 %.
  
  

Latest revision as of 11:48, 10 October 2022


EXP-nCBE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 745
    Illegal NheI site found at 2227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5551
    Illegal BamHI site found at 4506
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1118
    Illegal BsaI site found at 6129
    Illegal BsaI.rc site found at 1112
    Illegal BsaI.rc site found at 5238
    Illegal BsaI.rc site found at 6393


Design Notes

A cytosine base editor with EXP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 70 %.


Source

NONE

References