Difference between revisions of "Part:BBa K4192022"
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It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens. | It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens. | ||
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+ | ===Usage=== | ||
+ | Basic part <i>FpOAR</i>, encoding the efflux channel of oxalate.In our project, we used this part to efflux oxalic acid produced by engineering bacteria to achieve extracellular enrichment of oxalic acid. After exogenously adding calcium ions, calcium oxalate protective film was formed. | ||
+ | ===Biology=== | ||
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+ | ===Characterization=== | ||
+ | The part comes from <i>Fomitopsis palustris</i> originally, therefore we need to test if the gene FpOAR can work in prokaryotes as well | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 11:24, 10 October 2022
FpOAR, oxalate efflux channel protein
It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens.
Usage
Basic part FpOAR, encoding the efflux channel of oxalate.In our project, we used this part to efflux oxalic acid produced by engineering bacteria to achieve extracellular enrichment of oxalic acid. After exogenously adding calcium ions, calcium oxalate protective film was formed.
Biology
Characterization
The part comes from Fomitopsis palustris originally, therefore we need to test if the gene FpOAR can work in prokaryotes as well Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 158
Illegal PstI site found at 301 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 158
Illegal PstI site found at 301 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 158
Illegal BglII site found at 1161
Illegal XhoI site found at 973 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 158
Illegal PstI site found at 301 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 158
Illegal PstI site found at 301 - 1000COMPATIBLE WITH RFC[1000]