Difference between revisions of "Part:BBa K215260"
| Line 2: | Line 2: | ||
<partinfo>BBa_K215260 short</partinfo> | <partinfo>BBa_K215260 short</partinfo> | ||
| − | This is a fusion protein that combines Lpp, OmpA, and WT streptavidin for the purpose of displaying streptavidin on the surface of the cell. TEV protease site included for easily removal of strepavidin (and any other proteins bound to streptavidin) by treatment with TEV protease. | + | This is a fusion protein that combines Lpp, OmpA, and WT streptavidin for the purpose of displaying streptavidin on the surface of the cell. Lpp sequence targets protein to the periplasm, and OmpA transmembrane helix anchors it into the outer membrane with WT strepavidin on the extracellular side of the outer membrane. TEV protease site included for easily removal of strepavidin (and any other proteins bound to streptavidin) by treatment with TEV protease. |
See BBa_K215210 for the full inducible WT streptavidin display system. | See BBa_K215210 for the full inducible WT streptavidin display system. | ||
Latest revision as of 19:07, 1 October 2009
Lpp + 1tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His
This is a fusion protein that combines Lpp, OmpA, and WT streptavidin for the purpose of displaying streptavidin on the surface of the cell. Lpp sequence targets protein to the periplasm, and OmpA transmembrane helix anchors it into the outer membrane with WT strepavidin on the extracellular side of the outer membrane. TEV protease site included for easily removal of strepavidin (and any other proteins bound to streptavidin) by treatment with TEV protease.
See BBa_K215210 for the full inducible WT streptavidin display system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 256
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 298
Illegal AgeI site found at 349 - 1000COMPATIBLE WITH RFC[1000]