Difference between revisions of "Part:BBa K215210"
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<partinfo>BBa_K215210 short</partinfo> | <partinfo>BBa_K215210 short</partinfo> | ||
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This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG. | This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG. | ||
| − | Note: there's no T after this sequence and before the SpeI site. | + | Note: in order to express this construct as a fusion protein, there's no T after this sequence and before the SpeI site. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:57, 1 October 2009
IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin
This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
Note: in order to express this construct as a fusion protein, there's no T after this sequence and before the SpeI site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 337
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 379
Illegal AgeI site found at 430 - 1000COMPATIBLE WITH RFC[1000]