Difference between revisions of "Part:BBa K4175009"

 
 
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<partinfo>BBa_K4175009 short</partinfo>
 
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The CD3&#950; domain in chimeric antigen receptor (CAR) contains three immunoreceptor tyrosine-based activation motifs. Tyrosine phosphorylation in ITAM activates downstream signaling pathways. CD3 ITAMs are mainly phosphorylated by Lck and dephosphorylated by CD45. Diphosphorylated CD3 ITAMs can bind to Zap70 in tandem with SH2 domain to release Zap70 self-inhibition and activate downstream signaling pathways to activate T cells and secrete cytokines and cytotoxins to kill target cells.
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The CD3&#950; domain in chimeric antigen receptor (CAR) contains three immunoreceptor tyrosine-based activation motifs. Tyrosine phosphorylation in ITAM activates downstream signaling pathways. CD3 ITAMs are mainly phosphorylated by Lck and dephosphorylated by CD45. Dephosphorylated CD3 ITAMs can bind to Zap70 in tandem with SH2 domain to release Zap70 self-inhibition and activate downstream signaling pathways to activate T cells and secrete cytokines and cytotoxins to kill target cells. In our project, this part is used in anti-CD19 CAR (<partinfo>BBa_K4175012</partinfo>).
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4175009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4175009 SequenceAndFeatures</partinfo>
  

Latest revision as of 10:27, 10 October 2022


CD3-ζ domain

The CD3ζ domain in chimeric antigen receptor (CAR) contains three immunoreceptor tyrosine-based activation motifs. Tyrosine phosphorylation in ITAM activates downstream signaling pathways. CD3 ITAMs are mainly phosphorylated by Lck and dephosphorylated by CD45. Dephosphorylated CD3 ITAMs can bind to Zap70 in tandem with SH2 domain to release Zap70 self-inhibition and activate downstream signaling pathways to activate T cells and secrete cytokines and cytotoxins to kill target cells. In our project, this part is used in anti-CD19 CAR (BBa_K4175012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 239
  • 1000
    COMPATIBLE WITH RFC[1000]