Difference between revisions of "Part:BBa K4463007"

Revision as of 03:09, 9 October 2022 (view source) Qiaowa (Talk | contribs) ← Older edit		Latest revision as of 09:49, 10 October 2022 (view source) Qiaowa (Talk | contribs)
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−	The project of IGEM_XJTLU 2023 tested the adsorbed Cu(II) and Cd(II) concentration of part <a href="https://parts.igem.org/Part:BBa_K4463006">BBa_K4463006</a> and the part in this page.	+	The project of XJTLU_China 2022 tested the adsorbed Cu(II) and Cd(II) concentration of part <a href="https://parts.igem.org/Part:BBa_K4463006">BBa_K4463006</a> and the part in this page.
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−	For more information about our measure methods, please visit our wiki.	+	The results showed that we improved the original part successfully!
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		+	We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of <i>E.coli</i>. For detailed information about our measurement method and result analysis, please visit our <a href="https://2022.igem.wiki/xjtlu-china/experiments">Experiments</a>and <a href="https://2022.igem.wiki/xjtlu-china/results">Results</a>on wiki.
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Latest revision as of 09:49, 10 October 2022

SUMO-CsgA-ShMT3

CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and ShMT3. Improved parts is based on part BBa_K4463006.

The project of XJTLU_China 2022 tested the adsorbed Cu(II) and Cd(II) concentration of part BBa_K4463006 and the part in this page.

Figure1. The results showed that the ability of adsorbing Cu(II) by modified E. coli growing in 1.8mM CuSO4 is: SUMO-ShMT3> ShMT3> Control
Figure2. The results showed that the ability of adsorbing Cd(II) by modified E. coli growing in 300μM CdCl2 is: SUMO-ShMT3> ShMT3> Control

The results showed that we improved the original part successfully!

We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of E.coli. For detailed information about our measurement method and result analysis, please visit our Experimentsand Resultson wiki.

Sequence and Features