Difference between revisions of "Part:BBa K4375014"
Line 7: Line 7:
 
==Usage and Biology==
 
==Usage and Biology==
  
−
The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor.
+
The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification ot co-immunoprecipitation.  
−
A Myc-tag can be used to detect expression of recombinant proteins.
+
−
First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.
+
  
  
Line 17: Line 15:
 
<partinfo>BBa_K4375014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4375014 SequenceAndFeatures</partinfo>
  
−
==References==
+
==Reference==
  
−
https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/
+
„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/  
  
  

Revision as of 09:31, 10 October 2022


Myc Tag

Myc Tag derived from c-Myc.

Usage and Biology

The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification ot co-immunoprecipitation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/