Difference between revisions of "Part:BBa K4241791"

 
 
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<partinfo>BBa_K4241791 short</partinfo>
 
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<partinfo>BBa_K4241791 SequenceAndFeatures</partinfo>
  
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===Overview===
 
This part is CvNDI2, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9
 
This part is CvNDI2, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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CvNDI2 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
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This component is a promoter region to be added before the coding sequence of the protein of interest. The activation of the promoter must be induced via nitrogen starvation.
  
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===References===
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<span class='h3bb'>Sequence and Features</span>
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Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9
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<partinfo>BBa_K4241791 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 09:31, 10 October 2022


Nitrogen Starvation Inducible promoter 2 (Microalgae)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 87
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 238
    Illegal XhoI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

This part is CvNDI2, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

CvNDI2 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression. This component is a promoter region to be added before the coding sequence of the protein of interest. The activation of the promoter must be induced via nitrogen starvation.

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9