Difference between revisions of "Part:BBa K4241790"

 
 
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<partinfo>BBa_K4241790 short</partinfo>
 
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<partinfo>BBa_K4241790 SequenceAndFeatures</partinfo>>
  
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Nitrogen starvation inducible promoter 1 found from chlorella found from https://www.nature.com/articles/s41598-020-69620-9
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===Overview===
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This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
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<br/>
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This component is a promoter region to be added before the coding sequence of the protein of interest. The activation of the promoter must be induced via nitrogen starvation.
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===References===
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Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., &amp; Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4241790 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 09:24, 10 October 2022


Nitrogen Starvation Inducible promoter 1 (Microalgae)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
>

Overview

This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
This component is a promoter region to be added before the coding sequence of the protein of interest. The activation of the promoter must be induced via nitrogen starvation.

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9